Gut microbes play an important role in regulating host physiology and metabolism. Recent studies indicate that gut microbes affect memory formation and neurodegenerative disease manifestation. However, it is not fully understood how gut microbes influence gut?brain communication. The nematode C. elegans has a relatively simple nervous system and will readily ingest microbes from a petri dish. Therefore, C. elegans is a well-established model organism for studying the neural basis of behaviors and host?microbe interactions. By combining the two areas of research, we recently discovered that superoxide dismutase-1 (SOD-1), an enzyme that converts superoxide to less harmful peroxide, acts as a reactive sensor of reactive oxygen species in the nervous system and regulates the behavioral response to microbes. Our data indicate a previously unknown function of SOD-1 and suggest a potential role for SOD-1 in regulating microbe-induced gut?brain communication.
In aim 1, we will determine how bacteria activate SOD-1.
In aim 2, we will determine whether a neuropeptide functions as a signaling molecule between the gut and the nervous system to modulate the SOD-1 dependent response.
In aim 3, we will define how SOD-1 and glutamatergic signaling regulate behavioral response to microbes. The proposed work will advance our knowledge in several ways. Our genetic model will open up a new research area for understanding the microbe-induced communication at the gut ?brain axis. In addition, t he proposed experiments will unveil new regulatory pathways of SOD-1. Our results may help to elucidate the underlying mechanisms of gut dysbiosis among elderly adults and patients with neurodegenerative diseases and may lead to the identification of potential therapeutic target.

Public Health Relevance

The proposed research will employ a host?microbe model to investigate oxidative, neuroendocrine, and glutamatergic responses at the microbe-gut-neuron interface. The results of the proposed project may lead to discovery of novel molecular mechanisms and targets to ameliorate symptoms of gut dysbiosis, which is often observed in the aging population and neurodegenerative disease patients.

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Research Project (R01)
Project #
7R01GM131156-02
Application #
10114671
Study Section
Neural Oxidative Metabolism and Death Study Section (NOMD)
Program Officer
Maas, Stefan
Project Start
2019-08-01
Project End
2024-05-31
Budget Start
2020-01-20
Budget End
2020-05-31
Support Year
2
Fiscal Year
2019
Total Cost
Indirect Cost
Name
Rowan University School/Osteopathic Med
Department
Anatomy/Cell Biology
Type
Schools of Osteopathic Medicine
DUNS #
078789801
City
Stratford
State
NJ
Country
United States
Zip Code
08084